Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis.
نویسندگان
چکیده
Peptide nucleic acid (PNA) probes were designed to bind to the internal reference sequence and the single base mutation sequence within PCR-amplified DNA templates. PNAs hybridized to the target sequences on DNA were analyzed using MALDI-TOF mass spectrometry. Accurate quantification of the relative amount of mutant DNA was reproducibly demonstrated.
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عنوان ژورنال:
- Chemical communications
دوره 49 36 شماره
صفحات -
تاریخ انتشار 2013